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Anti-SARS-CoV-2 Nucleocapsid antibody(DM22), Rabbit mAb
Clone ID | DM22 |
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Immunogen | Recombinant SARS-CoV-2 Nucleocapsid (Met1-Ala 419) (PME100459) produced by using E. coli |
Buffer | 1XPBS |
Catalog Number | DME100015 |
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Package | 10μg ¥630.00 ; 50μg ¥1880.00 ; 100 μg ¥3380.00 ; 500 μg ¥11080.00 |
Delivery | In Stock |
Synonyms | Nucleocapsid, N protein, protein N,SARS-CoV-2,SARS |
Host Species | Rabbit |
IgG type | Rabbit IgG |
Clonality | Monoclonal |
Formulation | Liquid |
Purification | Purified from cell culture supernatant by affinity chromatography |
Reactivity | SARS-CoV-2 |
Applications | ELISA |
Recommended Dilutions | ELISA 1/5000-1/10000 |
Storage | Store at -20°C for 12 months (Avoid repeated freezing and thawing) |
Usage | Research use only |
Images |
Figure 1. Elisa plate pre-coated by 2 μg/ml(100μl/well) SARS-CoV-2 Nucleocapsid protein, His Tag(Cat.No.PME100459) can bind Rabbit Anti-SARS-CoV-2 Nucleocapsid monoclonal antibody(clone:DM22) in a linear range of 0.24-62.5 ng/ml. |
Background | Coronavirus contain most of nucleocapsid protein. Coronavirus nucleoproteins (N proteins) localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. The nucleolus is the site of ribosome biogenesis and sequesters cell cycle regulatory complexes. Two of the major components of the nucleolus are fibrillarin and nucleolin. These proteins are involved in nucleolar assembly and ribosome biogenesis and act as chaperones for the import of proteins into the nucleolus. Regarding of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is a tool for diagnostic. |
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