Clone ID | DM53 |
---|---|
Target | |
Synonyms | B7-H3; CD276; B7 homolog 3; B7H3 |
Host Species | Rabbit |
Description | Anti-B7-H3 antibody(DM53); Rabbit mAb |
Delivery | In Stock |
Uniprot ID | Q5ZPR3 |
IgG type | Rabbit IgG |
Clonality | Monoclonal |
Reactivity | Human |
Applications | ELISA; Flow Cyt |
Recommended Dilutions | ELISA 1:5000-10000; Flow Cyt 1:100 |
Purification | Purified from cell culture supernatant by affinity chromatography |
Formulation & Reconstitution | Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
Storage & Shipping | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
Background | The protein encoded by this gene belongs to the immunoglobulin superfamily; and thought to participate in the regulation of T-cell-mediated immune response. Studies show that while the transcript of this gene is ubiquitously expressed in normal tissues and solid tumors; the protein is preferentially expressed only in tumor tissues. Additionally; it was observed that the 3' UTR of this transcript contains a target site for miR29 microRNA; and there is an inverse correlation between the expression of this protein and miR29 levels; suggesting regulation of expression of this gene product by miR29. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
Usage | Research use only |
Conjugate | Unconjugated |
Anti-B7-H3 antibody(DM53); Rabbit mAb
Price: 10μg ¥690.00 ; 50μg ¥2280.00 ; 100 μg ¥4080.00 ; 500 μg ¥13280.00
Product Data
图片
Figure 1. Flow cytometry data of serially titrated Rabbit anti-B7H3 monoclonal antibody (clone: DM53) on on Expi 293 cell line transfected with human B7-H3. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.
Figure 2. Expi 293 cell line transfected with irrelevant protein (A) and human B7-H3 (B) were surface stained with Rabbit anti-B7-H3 monoclonal antibody 1μg/ml (clone: DM53) followed by Alexa 488-conjugated anti-rabbit IgG secondary antibody.
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