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Human CTLA-4 Protein, hFc tag

产品概述
Catalog Number PME100479
Package 10 μg ¥800.00 ; 50 μg ¥3100.00 ; 100 μg ¥4600.00
Description Recombinant human CTLA-4 protein with C-terminal human Fc tag
Synonyms CTLA4, CD152
Delivery In Stock
Uniprot ID P16410
Expression Host HEK293
Tag C-Human Fc
Molecular Characterization 这里上传图片
Molecular Weight The protein has a predicted molecular mass of 39.6 kDa after removal of the signal peptide.The apparent molecular mass of CTLA4-hFC is approximately 40-55 kDa due to glycosylation.
Purity The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Formulation & Reconstitution Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions.
Storage Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing).lyophilized proteins are shipped at ambient temperature.
Usage Research use only
Images

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Figure 1. Human CTLA-4 Protein, hFc Tag on SDS-PAGE under reducing condition.

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Figure 2. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CTLA4, hFc tagged protein (PME100479) can bind Anti-CTLA4 Neutralizing antibody(BME100022) in a linear range of 0.64-80.0 ng/ml.

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Figure 3. HEK293 cell line transfected with irrelevant protein (B) and human B7-2 (A) were surface stained with Human CTLA4, hFc tagged protein (PME100479) 1μg/ml followed by Alexa 488-conjugated anti-human IgG secondary antibody.

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Figure 4. Flow cytometry analysis with 1ug/ml Human CTLA4 Protein, hFc tag (PME100479) on Expi293 cells transfected with human B7-1 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).


Background This gene is a member of the immunoglobulin superfamily and encodes a protein which transmits an inhibitory signal to T cells. The protein contains a V domain, a transmembrane domain, and a cytoplasmic tail. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. The membrane-bound isoform functions as a homodimer interconnected by a disulfide bond, while the soluble isoform functions as a monomer. Mutations in this gene have been associated with insulin-dependent diabetes mellitus, Graves disease, Hashimoto thyroiditis, celiac disease, systemic lupus erythematosus, thyroid-associated orbitopathy, and other autoimmune diseases.
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