Recombinant human NEFL(89-400) protein with N-terminal 6×His tag
6×His tag NEFL(Gln89-Leu400)
The protein has a predicted molecular mass of 36.9 kDa after removal of the signal peptide. The apparent molecular mass of His-NEFL(89-400) is approximately 35-55 kDa due to glycosylation.
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining.
|Formulation & Reconstitution||
Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
|Storage & Shipping||
Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y. [provided by RefSeq, Oct 2008]
Research use only
Human NEFL (89-400) Protein, His Tag
Price: 10μg ￥782.00 ; 50μg ￥2866.00 ; 100 μg ￥4299.00
Figure 1. Human NEFL(89-400) Protein, His Tag on SDS-PAGE under reducing condition.
Figure 2. ELISA plate pre-coated by 1 μg/mL (100 μL/well) Human NEFL (89-400) Protein, His Tag (PME100648) can bind Anti-NEFL(89-400) antibody(DM199), Rabbit mAb (DME100199) in a linear range of 0.128-80 ng/mL.