| Target | |
|---|---|
| Synonyms | TIGIT;VSIG9;VSTM3 |
| Description | Recombinant human TIGIT Protein with C-terminal human Fc tag |
| Delivery | In Stock |
| Uniprot ID | Q495A1 |
| Expression Host | HEK293 |
| Tag | C-Human Fc Tag |
| Molecular Characterization | TIGIT(Met22-Pro141) hFc(Glu99-Ala330) |
| Molecular Weight | The protein has a predicted molecular mass of 39.13 kDa after removal of the signal peptide.The apparent molecular mass of TIGIT-hFc is approximately 40-53 kDa due to glycosylation. |
| Purity | The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. |
| Formulation & Reconstitution | Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| Storage & Shipping | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| Background | This gene encodes a member of the PVR (poliovirus receptor) family of immunoglobin proteins. The product of this gene is expressed on several classes of T cells including follicular B helper T cells (TFH). The protein has been shown to bind PVR with high affinity, this binding is thought to assist interactions between TFH and dendritic cells to regulate T cell dependent B cell responses. |
| Usage | Research use only |
Human TIGIT Protein, hFc Tag
Price: 10μg ￥818.00 ; 50μg ￥2999.00 ; 100 μg ￥4499.00
Product Data
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Figure 1. Human TIGIT Protein, hFc Tag on SDS-PAGE under reducing condition.
Figure 2. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CD155, mFc-His tagged protein () can bind Human TIGIT, hFc tagged protein (PME100107) in a linear range of 1.95-125 ng/ml.
Figure 3.Flow cytometry analysis with 1μg/mL Human TIGIT Protein, hFc tag (PME100107) on Expi293 cells transfected with human CD112 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).
Figure 4. ELISA plate pre-coated by 2 μg/mL (100 μL/well) Human TIGIT Protein, hFc Tag (PME100107) can bind Anti-TIGIT (etigilimab biosimilar) mAb (BME100024) in a linear range of 0.32–8 ng/mL.