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Human CD28 Protein, mFc-His Tag

产品概述
Catalog Number PME100014
Package 10 μg ¥800.00 ; 50 μg ¥3100.00 ; 100 μg ¥4600.00
Description Recombinant human CD28 protein with C-terminal mouse Fc and 6×His tag
Synonyms CD28, Tp44
Delivery In Stock
Uniprot ID P10747
Expression Host HEK293
Tag C-Mouse Fc and 6×His tag
Molecular Characterization 这里上传图片
Molecular Weight The protein has a predicted molecular mass of 68-72 kDa after removal of the signal peptide.
Purity The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Formulation & Reconstitution Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions.
Storage Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing).Lyophilized proteins are shipped at ambient temperature.
Usage Research use only
Images

Figure 1. Human CD28 Protein, mFc-His Tag on SDS-PAGE under reducing condition.

Figure 2. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human B7-1, hFc tagged protein (PME100473) can bind Human CD28, mFc-His tagged protein (PME100014) in a linear range of 125-4000 ng/ml.

Figure 3. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human B7-2, hFc tagged protein (PME100474) can bind Human CD28, mFc-His tagged protein (PME100014)  in a linear range of 62.5-4000 ng/ml.

Figure 4. HEK293 cell line transfected with irrelevant protein (B) and human B7-2 (A) were surface stained with Human CD28, mFc-His tagged protein (PME100014) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

Background The protein encoded by this gene is essential for T-cell proliferation and survival, cytokine production, and T-helper type-2 development. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene.
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