Human FLT3 Protein, hFc-His Tag
|Package||10 μg ￥700.00 ; 50 μg ￥2800.00 ; 100 μg ￥4100.00|
|Description||Recombinant human FLT3 protein with C-terminal human Fc and 6×His tag|
|Synonyms||Flt-3, Flk-2, STK-1, CD135, FLK2, FLT-3|
|Tag||C-Human Fc and 6xHis tag|
|Molecular Weight||The protein has a predicted molecular mass of 125-130 kDa after removal of the signal peptide.|
|Purity||The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.|
|Formulation & Reconstitution||Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions.|
|Storage||Store at -20°C to -70°C for 12 months in lyophilized state|
|Usage||Research use only|
Figure 1. Human FLT3, hFc-His Tag on SDS-PAGE under reducing condition.
Figure 2. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human FLT3LG, mFc-His tagged protein (PME100033) can bind Human FLT3, hFc-His tagged protein (PME100007) in a linear range of 0.128-10.02 ng/ml.
|Background||Flt-3(Receptor-type tyrosine-protein kinase FLT3) is also known as FLK-2(Fetal liver kinase-2), STK-1(Stem cell tyrosine kinase 1), CD135. FLT3 is a cytokine receptor which belongs to the receptor tyrosine kinase class III. Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine FLT3LG and regulates differentiation, proliferation and survival of hematopoietic progenitor cells and of dendritic cells. Promotes phosphorylation of SHC1 and AKT1, and activation of the downstream effector MTOR. Promotes activation of RAS signaling and phosphorylation of downstream kinases, including MAPK1/ERK2 and/or MAPK3/ERK1. Mutations that cause constitutive kinase activity promote cell proliferation and resistance to apoptosis via the activation of multiple signaling pathways.|