Anti-BTN3A1 antibody(DM94), Rabbit mAb
Recombinant human BTN3A1 (Glu30-Gly254) (PME100056) produced by using human HEK293 cells
|Buffer||Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions.|
|Package||10 μg ￥600.00 ; 100 μg ￥3880.00|
|Synonyms||BTN3A1, BTF5, CD277, BTN3.1, BT3.1|
|IgG type||Rabbit IgG|
|Purification||Purified from cell culture supernatant by affinity chromatography|
|Recommended Dilutions||ELISA 1/5000-10000;Flow Cyt 1/100|
|Storage||Store at -20°C for 12 months (Avoid repeated freezing and thawing)|
|Usage||Research use only|
Figure 1. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human BTN3A1 protein, mFc-His tagged protein (PME100056) can bind Rabbit anti-BTN3A1 monoclonal antibody (clone: DM94) in a linear range of 0.64-80 ng/ml.
Figure 2. Flow cytometry analysis with Anti-BTN3A1 (DM94) on Expi293 cells transfected with human BTN3A1 (Red histogram) or Expi293 transfected with irrelevant protein (Blue histogram).
|Background||The butyrophilin (BTN) genes are a group of major histocompatibility complex (MHC)-associated genes that encode type I membrane proteins with 2 extracellular immunoglobulin (Ig) domains and an intracellular B30.2 (PRYSPRY) domain. Three subfamilies of human BTN genes are located in the MHC class I region: the single-copy BTN1A1 gene (MIM 601610) and the BTN2 (e.g., BTN2A1; MIM 613590) and BTN3 (e.g., BNT3A1) genes, which have undergone tandem duplication, resulting in 3 copies of each.|