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Human IL5 Protein, His Tag

产品概述
Catalog Number PME100085
Package 10 μg ¥750.00 ; 50 μg ¥2900.00 ; 100 μg ¥4400.00
Description Recombinant Human IL5 protein with C-terminal 6×His tag
Synonyms IL-5, TRF, Interleukin-5, EDF
Delivery In Stock
Uniprot ID P05113
Expression Host HEK293
Tag C-6×His tag
Molecular Characterization 这里上传图片
Molecular Weight The protein has a predicted molecular mass of 13.94 kDa after removal of the signal peptide.The apparent molecular mass of IL5-His is approximately 15-25 kDa due to glycosylation.
Purity The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Formulation & Reconstitution Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions.
Storage Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing).
Usage Research use only
Images

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Figure 1. Human IL5 Protein, His Tag on SDS-PAGE under reducing condition.

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Figure 2. Flow cytometry analysis with 1 μg/mL Human IL5 Protein, His Tag (PME100085) on Expi293 cells transfected with Human IL5RA protein (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).

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Figure 3. ELISA plate pre-coated by 5 μg/mL (100 μL/well) Human IL5 protein, His Tag (PME100085) can bind Human IL5RA Protein, hFc Tag (PME100536) in a linear range of 6.4-4000 ng/mL.

Background This gene encodes a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. The encoded cytokine plays a major role in the regulation of eosinophil formation, maturation, recruitment and survival. The increased production of this cytokine may be related to pathogenesis of eosinophil-dependent inflammatory diseases. This cytokine functions by binding to its receptor, which is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF). This gene is located on chromosome 5 within a cytokine gene cluster which includes interleukin 4 (IL4), interleukin 13 (IL13), and CSF2 . This gene, IL4, and IL13 may be regulated coordinately by long-range regulatory elements spread over 120 kilobases on chromosome 5q31.
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