Anti-CD22 antibody(DM13), Rabbit mAb
Clone ID |
DM13 |
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Immunogen |
Recombinant human CD22 (Asp20-Arg687) (PME100005) produced by using human HEK293 cells |
Buffer | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions. |
Catalog Number | DME100013 |
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Package | 10 μg ¥600.00 ; 100 μg ¥3880.00 |
Delivery | In Stock |
Synonyms | SIGLEC-2, SIGLEC2 |
Host Species | Rabbit |
IgG type | Rabbit IgG |
Clonality | Monoclonal |
Formulation | Powder |
Purification | Purified from cell culture supernatant by affinity chromatography |
Reactivity | Human |
Applications | ELISA, Flow Cyt,WB, IP, IF |
Recommended Dilutions | Flow Cyt 1/100 |
Storage | Store at -20°C for 12 months (Avoid repeated freezing and thawing) |
Usage | Research use only |
Images |
Figure 1. Expi 293 cell line transfected with irrelevant protein (left) and human CD22 (right) were surface stained with Rabbit anti-CD22 monoclonal antibody 1μg/ml (clone: DM13) followed by Alexa 488-conjugated anti-rabbit IgG secondary antibody.
Figure 2. Flow cytometry data of serially titrated Rabbit anti-CD22 monoclonal antibody (clone: DM13) on Raji cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.
Figure 3. Affinity ranking of different Rabbit anti-CD22 mAb clones by titration of different concentration onto Raji cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.
Figure 4. Phylogenetic analysis of amino acid sequence of different Rabbit Anti-CD22 mAb clones. A) Heavy chain and B) Light chain. |
Background | CD22 (CD22 Molecule) is a Protein Coding gene. Diseases associated with CD22 include Refractory Hematologic Cancer and Hairy Cell Leukemia. Among its related pathways are Downstream signaling events of B Cell Receptor (BCR) and Hematopoietic cell lineage. Gene Ontology (GO) annotations related to this gene include carbohydrate binding. An important paralog of this gene is SIGLEC2. |
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